organs under sterile conditions on a nutrient culture medium of known composition. Plant develoment is charactrized by the continuous initiation of tissues and organ. The meristem, which are small stem cell population, are involved in this process. The in vitro propagation of explants is influenced by cytokinin which makes its concentration adjustment necessary in order to optimize the multiplication rate . Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or used was consisted by macronutrients and micronutrients of MS medium. Nodal segment explants were excised from shoots cultured in MS medium without BAP, inoculated in flasks containing 30 ml of culture .Therefore, the plant cell and tissue require a proper medium for their growth and developmant . The nutrient media for plant cell is a well defined mixture of inorganic salts and typical carbon sources like sucrose and glucose. In majority of cell, supplementary constituents are required which are the essential growth regulators i. e. indole acetic acid (IAA), kinetin and NAA, vitamins like thiamin and nicotinic acid, amino acids, inositol and sugar alcohols.
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